VETSCAN HM5
Sample collection
Keys to successful sample collection
The quality of the sample analyzed is directly related to the quality of the result.
- Minimize suction on the syringe, and do not draw back too quickly
- Use the largest vein and needle appropriate for blood collection
- Avoid use of any needle smaller than a 23 gauge (though certain exotic species may require a smaller needle)
- Use minimal alcohol on fur/skin
- Remove the needle from the syringe before dispensing into the blood tube unless using a closed vacuum blood collection system
- Fill EDTA tube to manufacturer’s sample fill line
- Immediately after filling tube, cap the tube and invert 15-20 times to sufficiently mix with anticoagulant (more inversions would be needed in case of 0.25 mL, 0.5 mL or 1.3 mL tubes)

- Select tubes based on the testing requirements and size of patient (Microtainer®, 1.3 mL, 3 mL, 5 mL)
- Ensure tubes have not expired
- Always fill blood tubes in the correct order to avoid contamination
- EDTA (ethylenediaminetriacetic acid) contamination of chemistry samples may affect electrolyte results and cause a falsely low Ca (calcium) and falsely high K+ (potassium)
- If improper tube-filling order occurs, the sample should be redrawn
- Do not hold off the vein for more than a few seconds before venipuncture
- For feline samples collected from the medial saphenous vein, a vacuum blood collection system instead of a syringe is recommended
- Run samples as soon as possible after drawing
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* If you are a pet owner looking for treatment recommendations, please contact your veterinarian.